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Screen Quest膜电位分析试剂盒(橙色荧光)
面议Screen Quest Membrane Potential Assay Kit Orange F
面议AAT Bioquest Screen Quest膜电位分析试剂盒(橙色荧光)
面议AAT Bioquest Screen Quest Membrane Potential Assay
面议Screen Quest膜电位检测试剂盒 红色荧光
面议Screen Quest Membrane Potential Assay Kit Red Fluo
面议AAT Bioquest Screen Quest膜电位检测试剂盒 红色荧光
面议AAT Bioquest Screen Quest Membrane Potential Assay
面议Screen Quest膜电位检测试剂盒红色荧光
面议Screen Quest Membrane Potential Assay Kit Red Fluo
面议AAT Bioquest Screen Quest膜电位检测试剂盒红色荧光
面议AAT Bioquest Screen Quest Membrane Potential Assay
面议产品描述:
蛋白酶体zui通用的形式就是26s蛋白酶体,它包含一个20S的核心结构颗粒和两个19S调节调节基团。所有20S的核心结构颗粒包含四对七聚物环状结构组成两种不同的亚基,alpha亚基是结构亚基,beta亚基主要是催化亚基。在两个环状结构外每个含有七个alpha亚基主要是为了调整颗粒的停靠。Alpha亚基N端结构是可以阻挡未调节底物进入内腔的门户。内腔两个环分别包含七个beta亚基包含酶激活位点来执行蛋白水解反应。Amplite 20S蛋白酶体荧光检测试剂盒可以用来检测培养细胞或者细胞裂解液里蛋白酶体复合物中类似*蛋白酶的活性。这个试剂盒使用LLVY-R110作为蛋白酶的荧光指示剂。通过蛋白酶切开LLVY-R110产生强绿色的R110,其检测荧光在520-530nm,激发光为480-500nm。该试剂盒提供所有必要成分并提供*实验操作方案。这个实验能稳定并高通量的评估从培养细胞或者溶液中蛋白酶体的活性和筛选抑制剂。该实验方案能方便的在96或384微孔板中分析。
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英文注释:
Our Amplite Fluorometric 20S Proteasome Assay Kit is a homogeneous fluorescent assay that measures the chymotrypsin-like protease activity associated with the proteasome complex either in cultured cells or cell lysates. This kit uses LLVY-R110 as a fluorogenic indicator for proteasome activity. Cleavage of LLVY-R110 by proteasome generates the strongly green fluorescent R110 that is monitored fluorimetrically at 520-530 nm with excitation of 480-500 nm. The kit provides all the essential components with an optimized assay protocol. The assay is robust, and can be readily adapted for high-throughput assays for evaluation of proteasome activity or inhibitor screening in cultured cells or in solution. The assay can be performed in a convenient 96-well and 384-well fluorescence microtiter-plate format. The main function of the proteasome is to degrade unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds. The proteasomal degradation pathway is essential for many cellular processes, including the cell cycle, the regulation of gene expression, and responses to oxidative stress. The most common form of the proteasome in this pathway is the 26S proteasome, an ATP-dependent proteolytic complex, which contains one 20S (700-kDa) core particle structure and two 19S (700-kDa) regulatory caps. The 20S core contains three major proteolytic activities including chymotrypsin-like, trypsin-like and caspase-like. It is responsible for the breakdown of key proteins involved with apoptosis, DNA repair, endocytosis, and cell cycle control.
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Screen Quest Fluo-4钙检测试剂盒 *去除培养基* | 36331 | 1 kit |
Screen Quest Fluo-4钙检测试剂盒 *去除培养基* | 36332 | 1 kit |
Screen Quest 免洗Fluo-4钙流分析试剂盒 *1% FBS生长培养基* | 36334 | 1 kit |
Screen Quest Fluo-4钙检测试剂盒 *1% FBS生长培养基* | 36335 | 1 kit |
台盼蓝钠盐 *细胞培养验证* | 2450 | 100 g |
台盼蓝钠盐 *超级纯* *不含荧光杂质* | 2452 | 10 g |
Trypan Blue, 钠盐 *溶解于10 mM水溶液中* | 2453 | 10 mL |
台盼超级蓝钠盐 *0.1M水溶液* | 2455 | 1 mL |
台盼红 Plus *0.1 M水溶液* | 2456 | 10 mL |
台盼红 Plus *0.1 M水溶液* | 2457 | 100 mL |
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台盼紫 *0.1M水溶液* | 2466 | 100 mL |
Screen Quest Fluo-8钙检测试剂盒 | 36314 | 10plates |
钙离子荧光探针Fluo-4, AM | TJ2701 | 100 μg |
Cal Green™ 1, AM [Equivalent to Calcium Green -1, AM] | 20502 | 1 mg |
Quest Rhod-4™, 钠盐(Quest Rhod-4™, sodium salt ) | 21118 | 1 mg |
Quest Rhod-4™, 钾盐(Quest Rhod-4™, potassium salt) | 21119 | 1 mg |