Anti-M13 antibody [B62-FE2] HRP

From:ABCAM

Catalog Number : ab50370

Quantity size :   0.1ml  （dilute with pH 7.4    0.01 M PBS or antibody diluent ） Relevance: The bacteriophage known as "M13" forms the basis of cloning systems designed to easily introduce mutations into genes inserted into the phage genome. It also has been used in various "phage display" methodologies and combinatorial DNA and peptide libraries. Coat protein A （G3P） is necessary for adsorption of the virion onto the F-pilus of the host cell. There are about five copies of this protein per mature phage. Consists of three domains （N1, N2, and CT）. The N2 domain interacts with the F pilus, whereas the N1 domain （connected to N2 by a flexible glycine-rich linker and tightly interacting with it on the phage） forms a complex with the C-terminal domain of tolA at later stages of the infection process. They are located at the adsorption end of the phage particle.

.Specificity : Anti- M13 is a mouse monoclonal antibody HRP           

specific for M13of hu

use for western blotting,elisa,immunoprecipitation and immunohistochemistry

• Protein G affinity chromatography purification, purity :>95%
• Isotype:  IgG
• mol wt:  130kDa
• Application :  
•  WB=1:50-200  ELISA=1:100-1000  IHC-P=1:50-200  IHC-F=1:50-200
• not yet tested in other applications.
  optimal dilutions/concentrations should be determined by the end user..

Storage:  Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The  lyophilized antibody is stable at room temperature for at least one month and for greater  than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent  of antibody, the antibody is stable for at least six weeks at 2-4 ℃

Important Note: This product as supplied is intended for research use only, not for use in  human, therapeutic or diagnostic applications.