Rabbit Anti-PI3K/PIK3R1抗体说明书下载
ABCAM

Cat. Number:  Rs-0128R

Quantity size:  0.1ml  （dilute with pH 7.4    0.01 M PBS or diluent of antibody）

Background:  The enzyme phosphatidylinositol 3 kinase （PI3 kinase） is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa （p110） and a regulatory p85 subunit （e.g. p85 alpha）. p85 alpha is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases （RTKs） through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 alpha is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.

Specificity:      ·  Anti-PI3K is a rabbit monoclonal antibody unconjugated

 ·  specific for PI3K of mouse 

· epitope mapping near the C terminus of PI3K of mouse 

·  use for western blotting, elisa, immunoprecipitation   

   and immunohistochemistry

·  Protein A affinity chromatography purification, purity :>95%

· Isotype: IgG   · mol wt: 48kDa

Application:     · Western blotting: 1:100-500

· Immunohistochemistry: 1:100-500

· ELISA: 1:500-1000

·  Optimal working dilutions must be determined by the end user.

Storage:   Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ºC.

Important Note:  This product as supplied is  intended for research use only, not for use in human, therapeutic or diagnostic applications.