Anti-CD4/Cy5标记抗体说明书

From:ABCAM

Catalog Number : RS-0766R-Cy5

Quantity size :   0.1ml  （dilute with pH 7.4    0.01 M PBS or antibody diluent ） Background: The CD4 antigen is highly expressed on human T helper cells and thymocytes, and at lower levels on monocytes and dendritic cells. This integral membrane glycoprotein of approximay 58 kDa contains four external domains （D1 to D4） that show homology to members of the immunoglobulin （Ig） superfamily. It is responsible for the recognition of the MHC class II antigen. The CD4 antibody recognizes most thymocytes and about 65% of peripheral blood T cells.
Subunit : Associates with p56-LCK. Interacts with SPG21.
Subcellular Location : Membrane; Single-pass type I membrane protein.
Similarity : Contains 3 Ig-like C2-type （immunoglobulin-like） domains.
Contains 1 Ig-like V-type （immunoglobulin-like） domain.

.Specificity : Anti- CD4/Cy5 is a rabbit polyclonal antibody              

specific forCD4/Cy5of  Mouse, Rat,

use for western blotting,elisa,immunoprecipitation and immunohistochemistry

• Protein G affinity chromatography purification, purity :>95%
• Isotype:  IgG
• mol wt:  48kDa
• Application :  
•  Flow-Cyt=1:50-200 
• IF=1:50-200
•  KLH conjugated synthetic peptide derived from the middle of mouse CD4
• not yet tested in other applications.
  optimal dilutions/concentrations should be determined by the end user..

Storage:  Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The  lyophilized antibody is stable at room temperature for at least one month and for greater  than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent  of antibody, the antibody is stable for at least six weeks at 2-4 ℃

Important Note: This product as supplied is intended for research use only, not for use in  human, therapeutic or diagnostic applications.