Anti-cox2抗体,wb,ihc抗体说明书
时间:2014-12-06 阅读:242
Rabbit Anti-cox2抗体,wb,ihc抗体说明书
From:ABCAM
Catalog Number : RS-6418R
Quantity size : 0.1ml (dilute with pH 7.4 0.01 M PBS or antibody diluent )
Background:COX2 (Cyclooxygenase 2) is an inducible enzyme. It is involved in the response of cells to growth factors, tumor promoters, and cytokines that induce its expression. Given its role in synthesizing prostaglandins, COX2 is therefore of interest in studying immune response regulation. COX2 is induced by a wide variety of stimuli and was initially identified as immediate early growth response gene. In addition, COX2 expression markedly increased in 85-90% of human colorectal adenocarcinoma whereas COX1 levels remain unchanged.
Specificity : Anti- cox2 is a rabbit polyclonal antibody
specific for cox2 of Rat ,hu,mo
- use for western blotting,elisa,immunoprecipitation and immunohistochemistry
- Protein G affinity chromatography purification, purity :>95%
- Isotype: IgG
- mol wt: 65kDa
Application :
- WB=1:100-500
- ELISA=1:500-1000
- IP=1:20-100
- IHC-P=1:100-500
- IHC-F=1:100-500
- IF=1:100-500
- optimal dilutions/concentrations should be determined by the end user..
Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.