Mouse Anti-phospho-NFKBp65(Ser536)实验
时间:2014-11-29 阅读:155
Mouse Anti-phospho-NFKBp65(Ser536)
From:abcam
Catalog Number : RS- 0982R
Quantity size : 100ul (dilute with pH 7.4 0.01 M PBS or antibody diluent )
background:
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011].
Specificity : Anti- phospho-NFKBp65(Ser536) is a mouse monoclonal antibody
specific for anti- phospho-NFKBp65(Ser536) of human,mouse, Rat ,
- use for western blotting,elisa,immunoprecipitation and immunohistochemistry
- Protein G affinity chromatography purification, purity :>95%
- Isotype: IgG
- mol wt: 61 kDa
Application :
- WB=1:100-500
- ELISA=1:500-1000
- IHC-P=1:100-500
- IHC-F=1:100-500
- optimal dilutions/concentrations should be determined by the end user..
KLH conjugated Synthesised phosphopeptide derived from human NFKBp65 around the phosphorylation site of Ser536
Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.