Anti-CD4/FITC

From:ABCAM

Catalog Number :Rs-0766R-FITC

Quantity size :   0.1ml (dilute with pH 7.4    0.01 M PBS or antibody diluent )Background:

This gene encodes a membraneglycoprotein of T lymphocytes that interacts with major histocompatibilitycomplex class II antigenes and is also a receptor for the humanimmunodeficiency virus. This gene is expressed not only in T lymphocytes, butalso in B cells, macrophages, and granulocytes. It is also expressed inspecific regions of the brain. The protein functions to initiate or augment theearly phase of T-cell activation, and may function as an important mediator ofindirect neuronal damage in infectious and immune-mediated diseases of thecentral nervous system. Multiple alternatively spliced transcript variantsencoding different isoforms have been identified in this gene. [provided byRefSeq, Aug 2010].Function : Accessory protein for MHC class-II antigen/T-cellreceptor interaction. May regulate T-cell activation. Induces the aggregationof lipid rafts.

Subunit : Associates with LCK. Bindsto HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, ispart of P4HB/PDI-CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelopepolyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsidproteins. Interacts with PTK2/FAK1; this interaction requires the presence ofHIV-1 gp120.Subcellular Location : Cell membrane; Single-pass type I membraneprotein. Note=Localizes to lipid rafts. Removed from plasma membrane by HIV-1Nef protein that increases clathrin-dependent endocytosis of this antigen totarget it to lysosomal degradation. Specificity : Anti-CD4/FITC  is a mouse Monoclonall antibody              

specific for CD4/FITC ofHuman,Rb

–    use for western blotting,elisa,immunoprecipitationand immunohistochemistry

–    Protein G affinity chromatographypurification, purity :>95%

–    Isotype:  IgG

–    mol wt: 48kDa

Application :  

–   Flow-cyt=1:50-200  IF=1:50-200

–   Excitation spectrum:520-570nm

–   Emission spectrum:490-510nm

–   not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user

Storage: Storeat -20℃ for oneyear. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least onemonth and for greater  than a year whenkept at -20℃.When reconstituted in sterile pH 7.4 0.01M PBS or diluent  of antibody, the antibody is stable for atleast six weeks at 2-4 ℃

Important Note: Thisproduct as supplied is intended for research use only, not for use in  human, therapeutic or diagnostic applications.